Anti-E. coli LacI [9A5]

Absolute Antibody

Anti-E. coli LacI [9A5]

9

The antibody recognizes LacI and recombinant proteins containing the LacI tag. The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. These genes are repress

This antibody was used for immunohistochemistry on tissue sections from mice transgenic for the R transgene (muscle, skin, hippocampus, cerebellum) (Cronin et al, 2001; PMID: 11410531). The antibody was used to detect the lac repressor protein by western blot analysis (Blevins et al., 2007; PMID: 17220265). The antibody was employed to stain third instar larval salivary glands (Hines et al., 2009; PMID: 19487560). Immunofluorescence staining using the antibody was performed on parental B49.5 lacO integrated cells and B49.5 cells stably expressing lacI-lap2b (Finlan et al., 2008; PMID: 18369458).

ImmunoFluorescence, Western Blot, ImmunoHistoChemistry

Research Use Only

Monoclonal

9A5

lacI

DNA-binding transcriptional repressor LacI

b0345; DNA-binding transcriptional repressor LacI; ECK0342

Rabbit

IgG

Lactose operon repressor

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Bacteria

-20°C,2°C to 8°C

12352203