Anti-E1 protein [DC1.7]

Absolute Antibody

Anti-E1 protein [DC1.7]

9

The binding characterization of the IgG antibody for CHIKV E1 protein was done using ELISA and Biolayer interferometry (BLI). This antibody was capable of binding biotinylated p62-E1 hybrid protein in a flow cytometric assay. This antibody was also capable of immunoprecipitating the E1 envelope protein of CHIKV. This antibody was can neutralize CHIKV 181/25 and CHIKV LR2006_OPY1 with IC50 value in the low nanomolar range and 13nM respectively. This antibody could not provide sufficient in vivo protection from CHIKV infection (PMID: 31697791).

Flow Cytometry, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

DC1.7

CHIKVgp2

structural polyprotein

CHIKVgp2, structural polyprotein

Mouse

IgG1

Structural polyprotein

Virus

-20°C,2°C to 8°C

12352203