Anti-Ebola GP [13F6-1-2]

Absolute Antibody

Anti-Ebola GP [13F6-1-2]

9

This antibody is specific for amino acids 401-417 of the ebola glycoprotein. The epitope has been mapped to nine residues (EQHHRRTDN, amino acids 405-413). Ebola is a viral hemorrhagic fever of humans and other primates caused by ebolaviruses.

This antibody recognizes the heavily glycosylated mucin-like domain of the Ebola virus virion-attached glycoprotein (GP) and protects animals against lethal viral challenge. To determine the protective efficacy of this antibody, the mouse version of this antibody was evaluated for their ability to protect mice from a lethal Ebola challenge. The characteristics were further analyzed using radioimmunoprecipitation, western blot, and peptide-binding analysis. These assays were performed using the mouse version of this antibody and Ebola GP as antigen. Furthermore, the ability of the mouse version of this antibody to inhibit the Ebola virus from forming plaques was also determined. This indicates that this antibody can neutralize the Ebola virus (US6875433). This antibody was expressed in plants, and to show its functionality, a western blot was performed with the mouse version of this antibody (which was expressed in a plant) on Ebola virus-like particles. Further, the mouse version of this antibody was also used for ELISA on Ebola VLP. Finally, to determine the binding affinity of the mouse version of this antibody, surface plasmon resonance was performed using Ebola VLP as an antigen (Lim et al., 2020; PMID: 32977599). To assess the specific binding of this antibody, an ELISA on Ebola GP was performed using the scFv version of this antibody (Rodriguez-Martinez et al., 2015; PMID: 26489048). The structure of the mouse version of this antibody was determined using molecular replacement. Further, the binding affinity of the mouse version of this antibody was determined using surface plasmon resonance on the Ebola virus bound to BSA (Lee et al., 2008; PMID: 18005986).

Functional Assay, Western Blot, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

13F6-1-2

GP

second secreted glycoprotein;small secreted glycoprotein;spike glycoprotein

second secreted glycoprotein;small secreted glycoprotein;spike glycoprotein; ZEBOVgp4

Human

IgG1

Envelope glycoprotein

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203