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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

Anti-ECD Antibody

HPA006465
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetECD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ECD
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11319
  • Target name
    ECD
  • Target description
    ecdysoneless cell cycle regulator
  • Target synonyms
    ecdysoneless homolog; GCR2; HSGT1; human suppressor of GCR two; protein ecdysoneless homolog; protein SGT1; SGT1; suppressor of GCR2; suppressor of S. cerevisiae gcr2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95905
  • Protein Name
    Protein ecdysoneless homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SGT1/ECD using anti-SGT1/ECD antibody (A00567-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse Ana-1 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGT1/ECD antigen affinity purified polyclonal antibody (Catalog # A00567-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGT1/ECD at approximately 73 kDa. The expected band size for SGT1/ECD is at 73 kDa.
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