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Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Anti-EDEM3 Antibody

HPA025755
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEDEM3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EDEM3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80267
  • Target name
    EDEM3
  • Target description
    ER degradation enhancing alpha-mannosidase like protein 3
  • Target synonyms
    C1orf22, CDG2V, ER degradation-enhancing alpha-mannosidase-like protein 3, ER degradation enhancer, mannosidase alpha-like 3, ER degradation-enhancing -mannosidase-like protein 3, ER degradation-enhancing alpha-mannosidase-like 3, alpha-1,2-mannosidase EDEM3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BZQ6
  • Protein Name
    ER degradation-enhancing alpha-mannosidase-like protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of EDEM3 using anti-EDEM3 antibody (A10297-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDEM3 antigen affinity purified polyclonal antibody (Catalog # A10297-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDEM3 at approximately 105 kDa. The expected band size for EDEM3 is at 105 kDa.
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