Figure 2. IHC analysis of EGFR using anti-EGFR antibody (A00023-2). EGFR was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-EGFR Antibody (A00023-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of EGFR using anti-EGFR antibody (A00023-2). EGFR was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-EGFR Antibody (A00023-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-EGFR Picoband(r) Antibody
A00023-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetEgfr
Overview
- SupplierBoster Bio
- Product NameAnti-EGFR Picoband(r) Antibody
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID13649
- Target nameEgfr
- Target descriptionepidermal growth factor receptor
- Target synonyms9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; epidermal growth factor receptor; Erb; Erbb; Err; Errb1; Errp; Wa; wa2; wa-2; Wa5; waved 2
- HostRabbit
- IsotypeIgG
- Protein IDQ01279
- Protein NameEpidermal growth factor receptor
- Scientific DescriptionBoster Bio Anti-EGFR Picoband® Antibody catalog # A00023-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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