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Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli.

Anti-EHD3 Antibody

HPA049986
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetEHD3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EHD3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID30845
  • Target name
    EHD3
  • Target description
    EH domain containing 3
  • Target synonyms
    EH domain-containing protein 3; PAST homolog 3; PAST3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZN3
  • Protein Name
    EH domain-containing protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EHD3 using anti-EHD3 antibody (A04576-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human CCRF-CEM whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human SY-SY5Y whole cell lysates, Lane 7: monkey heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHD3 antigen affinity purified polyclonal antibody (Catalog # A04576-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHD3 at approximately 70KD. The expected band size for EHD3 is at 70KD.
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