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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Anti-EIF3B Antibody

HPA048983
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEIF3B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EIF3B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8662
  • Target name
    EIF3B
  • Target description
    eukaryotic translation initiation factor 3 subunit B
  • Target synonyms
    EIF3-ETA, EIF3-P110, EIF3-P116, EIF3S9, PRT1, eukaryotic translation initiation factor 3 subunit B, eukaryotic translation initiation factor 3 subunit 9, eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD), eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa, protein synthesis 1, prt1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55884
  • Protein Name
    Eukaryotic translation initiation factor 3 subunit B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of EIF3B using anti-EIF3B antibody (A04318-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3B antigen affinity purified polyclonal antibody (Catalog # A04318-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF3B at approximately 115 kDa. The expected band size for EIF3B is at 92, 115 kDa.
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