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Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.

Anti-EIF4B Antibody

HPA039072
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetEIF4B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EIF4B
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1975
  • Target name
    EIF4B
  • Target description
    eukaryotic translation initiation factor 4B
  • Target synonyms
    EIF-4B; eukaryotic translation initiation factor 4B; PRO1843
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23588
  • Protein Name
    Eukaryotic translation initiation factor 4B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EIF4B using anti-EIF4B antibody (A00846-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4B antigen affinity purified polyclonal antibody (Catalog # A00846-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4B at approximately 80 kDa. The expected band size for EIF4B is at 80 kDa.
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