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Immunohistochemistry analysis in human appendix and cerebral cortex tissues using Anti-EML4 antibody. Corresponding EML4 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human appendix and cerebral cortex tissues using Anti-EML4 antibody. Corresponding EML4 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human appendix and cerebral cortex tissues using Anti-EML4 antibody. Corresponding EML4 RNA-seq data are presented for the same tissues.

Anti-EML4 Antibody

HPA065337
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEML4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EML4 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27436
  • Target name
    EML4
  • Target description
    EMAP like 4
  • Target synonyms
    C2orf2, ELP120, EMAP-4, EMAPL4, ROPP120, echinoderm microtubule-associated protein-like 4, EML4/ALK fusion protein, echinoderm microtubule associated protein like 4, restrictedly overexpressed proliferation-associated protein, ropp 120
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HC35
  • Protein Name
    Echinoderm microtubule-associated protein-like 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of EML4 using anti-EML4 antibody (A00930-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EML4 antigen affinity purified polyclonal antibody (Catalog # A00930-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EML4 at approximately 120KD. The expected band size for EML4 is at 109KD.
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