Anti-Endomucin [v.1A7.9]

Absolute Antibody

Anti-Endomucin [v.1A7.9]

9

This antibody is specific for carbohydrate epitopes of endomucin. The epitopes were resistant to endoglycosidase F. Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complex

In an attempt to identify the antigen to which this antibody binds multiple experiments were preformed. First, immunohistochemistry was preformed on normal mouse organs using the rat version of this antibody. Further, flow cytometry was preformed on mouse cells using the rat version of this antibody. Additionally, proteins were immunoprecipitated from lysed mouse cells using this antibody. And finally, the immunoprecipitated proteins were immunoblotted using the mouse version of this antibody (Morgan et al, 1999; pmid:9864158). To analyse the expression of endomucin during development of the vascular system immunohistochemistry was preformed on tissue from mouse embryos using the mouse version of this antibody (Brachtendorf et al, 2001; pmid:11747076). While characterizing KLEIPs function in ocular health and disease in mice this antibody was used for immunohistochemistry on the coronas of both KLEIP+/+ and KLEIP-/- mice (Hahn et al, 2012; pmid:22511632).

Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry

Research Use Only

Monoclonal

v.1A7.9

Emcn

endomucin

0610012K22Rik; AI315669; endomucin; endomucin-1; endomucin-1/2; Muc14; MUC-14; mucin 14, endothelial; mucin-14

Rabbit

IgG

Endomucin

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203