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Immunohistochemical staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.

Anti-ENPP1 Antibody

HPA062066
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetENPP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ENPP1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5167
  • Target name
    ENPP1
  • Target description
    ectonucleotide pyrophosphatase/phosphodiesterase 1
  • Target synonyms
    alkaline phosphodiesterase 1; ARHR2; COLED; ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Ly-41 antigen; M6S1; membrane component, chromosome 6, surface marker 1; NPP1; NPPS; PC-1; PCA1; PDNP1; phosphodiesterase I/nucleotide pyrophosphatase 1; plasma-cell membrane glycoprotein 1; plasma-cell membrane glycoprotein PC-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22413
  • Protein Name
    Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ENPP1/PC1 using anti-ENPP1/PC1 antibody (A01342-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENPP1/PC1 antigen affinity purified polyclonal antibody (Catalog # A01342-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENPP1/PC1 at approximately 130-150 kDa. The expected band size for ENPP1/PC1 is at 105 kDa.
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Lane 1: Human HepG2 cell lysates; Lane 2: Human Huvec cell lysates probed with ENPP1 Polyclonal Antibody, Unconjugated (bs-1760R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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