Anti-Envelope protein [ZKA78]

Absolute Antibody

Anti-Envelope protein [ZKA78]

9

ZKA78 is specific for EDI/II domain of the ZIKV envelope (E) protein, and cross-reacts with the E protein from Dengue virus (DENV), which is highly homologous (also contains high sequence similarity with other flaviviruses such as yellow fever and west ni

ZKA78 is able to partially neutralize ZIKV infectivity (IC50 ~2863 ng/ml), and is capable of enhancing ZIKV/ infection of K526 cells via the antibody-dependent enhancement (ADE) effect. This antibody can also neutralize (IC50~ 266 ng/ml) and enhance (by ADE) DENV infection. Neutralization of ZIKV infection can be measured using a micro-neutralization FC-based assay, and binding/specificity to E protein can be studied by ELISA. DENV2-infected AG129 mice administered with ZKA78 results in death after 5 days.

Flow Cytometry, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

ZKA78

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203