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Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Anti-EPHX2 Antibody

HPA023660
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEPHX2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EPHX2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2053
  • Target name
    EPHX2
  • Target description
    epoxide hydrolase 2
  • Target synonyms
    ABHD20, CEH, SEH, bifunctional epoxide hydrolase 2, epoxide hydratase, epoxide hydrolase 2, cytoplasmic, epoxide hydrolase 2, cytosolic, epoxide hydrolase, soluble
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP34913
  • Protein Name
    Bifunctional epoxide hydrolase 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EPHX2 using anti-EPHX2 antibody (A01999-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHX2 antigen affinity purified polyclonal antibody (Catalog # A01999-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHX2 at approximately 63 kDa. The expected band size for EPHX2 is at 63 kDa.
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