Anti-estrogen receptor alpha [D75]

Absolute Antibody

Anti-estrogen receptor alpha [D75]

9

Crude nuclear E*R of calf uterus (0.1 pmol), normal rat serum (10 Ml), and hybridoma medium were used in a double antibody immunoprecipitation with this antibody (Greene et al, 1980; pmid:6928610). ELISA was preformed with this antibody on human MCF-7 cell cytosol (Greene et al, 1982; pmid:6979662). Fresh breast cancer tumor was immunostained using this antibody (Miller et al, 1993; pmid:8490902). Sucrose Density Gradient Analysis was preformed on an extract made from MCF-7 human breast cancer cell line using this antibody (Wong et al, 1991; pmid:1705046). This antibody was used for immunohistochemistry on human breeast cancer (Wong et al, 1991; pmid:1705046). ELISA was preformed on human tumor whole-cell extract using this antibody (Scott et al, 1991; pmid:1864980). An immunohistochemistry was preformed on human breast cancer tissue using this antibody (Miller et al, 1993; pmid:8490902). Immunohistochemistry was preformed on tumor cells from humans with recurrent breast cancer. For this experiment this antibody was used (Rasmussen and Kamby, 1989; pmid:2616369). Western blot was preformed on SV40-transformed HBEC, and MCF-7, T47D, MDA-MB-231 cells using this antibody (Kang et al, 1997; pmid:9054615). Extracts from infected Sf9 cells were analyzed by Western blotting using this antibody (Beekman et al, 1993; pmid:8264659). Gel shift was preformed on human breast tumor sample and control MCF-7 extracts using this antibody (Montgomery et al, 1993; pmid:8219255).

Functional Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

D75

ESR1

estrogen receptor 1

E2 receptor alpha; ER; Era; ER-alpha; ESR; ESRA; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha

Mouse

IgG1

Estrogen receptor

-20°C,2°C to 8°C

12352203