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Immunohistochemical staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.

Anti-EVA1A Antibody

HPA008055
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEVA1A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EVA1A
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84141
  • Target name
    EVA1A
  • Target description
    eva-1 homolog A, regulator of programmed cell death
  • Target synonyms
    FAM176A; family with sequence similarity 176, member A; protein eva-1 homolog A; protein FAM176A; TMEM166; transmembrane protein 166
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H8M9
  • Protein Name
    Protein eva-1 homolog A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of EVA1A using anti-EVA1A antibody (A11580-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EVA1A antigen affinity purified polyclonal antibody (Catalog # A11580-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EVA1A at approximately 21KD. The expected band size for EVA1A is at 17KD.
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