Anti-F glycoprotein [12B2]

Absolute Antibody

Anti-F glycoprotein [12B2]

7

This antibody is reported to broadly neutralize nipah and hendra virus by blocking membrane fusion. Biolayer interferometry was used to characterize binding of the 12B2 Fab fragments to prefusion NiV F or HeV F ectodomain trimers immobilized on the surface of biosensors. The equilibrium dissociation constants (Kd) of 27.7 and 5.35 nM for attachment of Fab fragments of 12B2 to NiV F or HeV F, respectively. To further characterize this antibody, a kinetic assay was performed on HeV F- or NiV F- or NiV F S69A using the mouse version of this antibody. A cryo electron microscopy was performed on samples of the Fab fragment of this antibody bound to the F glycoprotein of the nipah virus (Dang et al, 2021; pmid:33927387). To analyze the ability of this antibody to prevent and cure disease, a fusion inhibition assay was performed on CHO745 cells transfected with HNV F and G glycoproteins using the mouse IgG version of this antibody. Further a plaque reduction assay was performed NiV-M, NiV-B or HeV using both the human and mouse IgG version of this antibody (Dang et al., 2021; PMID: 33927387).

Functional Assay, Neutralisation/Blocking

neutralize; functional assay

Research Use Only

-20°C,2°C to 8°C

12352203