Anti-F protein [mAb 234]

Absolute Antibody

Anti-F protein [mAb 234]

7

ELISA was used to confirm the binding of this antibody to hMPV F protein. This antibody has been reported to have high neutralizing activity against all of the hMPV types A1, A2, B1, and B2 with an in vitro IC50 of < 2microg/ml. The antibody is effective as passive prophylaxis, protecting hamsters and mice from hMPV challenge (PMID: 16873237).

ELISA, Neutralisation/Blocking

ELISA; neutralization

Research Use Only

Monoclonal

mAb 234

Mouse

IgM

Fusion glycoprotein F0

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Virus

The original antibody was generated by immunizing BALB/c mice with a combination of some or all of the following: intranasal infection with hMPV at 10^6 TCID50 per animal of either NL1700, NL100, or NL199; intranasal infection with 10^6 PFU b/hPIV3/hMPV FNL100; intraperitoneal injection with adenovirus-vectored hMPV FNL100 or hMPV FNL199 at a dose of 9 x 10^7 TCID50; purified soluble hMPV F protein derived from NL100 and NL199 sequences injected intraperitoneally with either GERBU MM adjuvant.

-20°C,2°C to 8°C

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