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Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.
Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.
Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.

Anti-F11 Antibody

HPA039808
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetF11
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-F11
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2160
  • Target name
    F11
  • Target description
    coagulation factor XI
  • Target synonyms
    FXI, PTA, coagulation factor XI, coagualtion factor XI, plasma thromboplastin antecedent
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP03951
  • Protein Name
    Coagulation factor XI
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of FXI/F11 using anti-FXI/F11 antibody (A00989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXI/F11 antigen affinity purified polyclonal antibody (Catalog # A00989-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FXI/F11 at approximately 150 kDa. The expected band size for FXI/F11 is at 70 kDa.
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