Anti-Fab (kappa) [TP1170]

Absolute Antibody

Anti-Fab (kappa) [TP1170]

9

This antibody recognises mouse kappa light chain.

Anti-IgG nanobodies can often perform more efficiently than polyclonal secondary antibodies in various experimental assays. For instance, this nanobody when conjugated with the infrared fluorophore IRDye 800 was more efficient in terms of the detection sensitivity of IgG2a antibodies than the polyclonal secondary antibody via Western blot (Pleiner et al., 2018). TP1170 can also be used as an efficient secondary nanobody in the indirect immunofluorescence. It was used in combination with other nanobodies to detect both mouse IgG1 and IgG2a primary antibodies giving strong, clean signal without any background staining (Pleiner et al., 2018). This nanobody is also highly recommended for superresolution microscopy, as it can place the fluorophore much closer to the antigen which had been bound by the primary antibody than a usual secondary antibody would be able to (Pleiner et al., 2018).

Dot Blot, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry

Research Use Only

Monoclonal

TP1170

Rabbit

IgG

This chimeric rabbit antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203