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anti-FADS1 antibody

ARG64123
Arigo Biolaboratories
ApplicationsWestern Blot
Product group Antibodies
TargetFADS1
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-FADS1 antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3992
  • Target name
    FADS1
  • Target description
    fatty acid desaturase 1
  • Target synonyms
    acyl-CoA (8-3)-desaturase; D5D; delta(5) desaturase; delta-5 fatty acid desaturase; FADS6; FADSD5; linoleoyl-CoA desaturase (delta-6-desaturase)-like 1; LLCDL1; TU12
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FADS1 using anti-FADS1 antibody (A02535-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADS1 antigen affinity purified polyclonal antibody (Catalog # A02535-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FADS1 at approximately 52 kDa. The expected band size for FADS1 is at 52 kDa.
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