Anti-FAK [3C4]

Absolute Antibody

Anti-FAK [3C4]

7

This antibody is specific for chicken tensin. Tensin may be involved in cell migration, fibrillar adhesion formation, cartilage development and in linking signal transduction pathways to the cytoskeleton.

To characterise the binding of the mouse version of this antibody to tyrosine-phosphorylated protein, immunoblotting on tyrosine-phosphorylated proteins from CEFs, NIH 3T3, Rat-1, HOS and 10W was performed. Furthermore, immunoprecipitating on the same samples was preformed. The antibody showed a reaction in both experiments and has cross-reactivity (Kanner et al, 1990; pmid:2110361). Cell lysates from CE cells infected with RCAS/RCAS-FRNK/RCAS-FAK were immunoprecipitated using the mouse version of this antibody. Furthermore, the immunoprecipitated proteins are western blotted using this antibody (Richardson and Parsons, 1996; pmid:8606775).

ImmunoPrecipitation, Western Blot

WB; IP

Research Use Only

Monoclonal

3C4

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Chicken

Chicken

The mouse version of this antibody was generated by immunizing mice with a mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src, and recognizes FAK.

-20°C,2°C to 8°C

12352203