Anti-FAK [5B9]

Absolute Antibody

Anti-FAK [5B9]

7

To study bacterial invasion. Lysates from 5637 cells infected with a bacterial suspension were immunoblotted using the mouse version of this antibody (Martinez et al, 2000; pmid:10856226). To study fibronectin matrix assembly. Western blot and immunoprecipitation was preformed on collagen-adherent FN-null cell monolayers. Furthermore FN-null MEFs were used for immunofluorescence (Lefort et al, 2011; pmid:21084302). Lysates from CE cells were both immunoprecipitated and blotted using the mouse version of this antibody. This was done to determine the wether CAKbeta shares properties with pp125FAK (Schaller and Sasaki, 1997; pmid:9312150).

ImmunoFluorescence, ImmunoPrecipitation, Western Blot

WB; IP; IF

Research Use Only

Monoclonal

5B9

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Chicken

Chicken

The mouse version of this antibody was generated by immunizing mice with a mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src, and recognizes FAK.

-20°C,2°C to 8°C

41116161