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Immunohistochemical staining of human tonsil shows weak to moderate nuclear positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows weak to moderate nuclear positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows weak to moderate nuclear positivity in germinal center cells.

Anti-FAM111B Antibody

HPA038637
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFAM111B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FAM111B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID374393
  • Target name
    FAM111B
  • Target description
    FAM111 trypsin like peptidase B
  • Target synonyms
    cancer-associated nucleoprotein; CANP; family with sequence similarity 111 member B; POIKTMP; serine protease FAM111B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6SJ93
  • Protein Name
    Protein FAM111B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FAM111B using anti-FAM111B antibody (A14246-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM111B antigen affinity purified polyclonal antibody (Catalog # A14246-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM111B at approximately 85 kDa. The expected band size for FAM111B is at 85 kDa.
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