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Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.

Anti-FAM50A Antibody

HPA003585
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFAM50A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FAM50A Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9130
  • Target name
    FAM50A
  • Target description
    family with sequence similarity 50 member A
  • Target synonyms
    9F, DXS9928E, HXC-26, HXC26, MRXSA, XAP5, protein FAM50A, Armfield X-linked mental retardation syndrome, protein XAP-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14320
  • Protein Name
    Protein FAM50A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FAM50A using anti-FAM50A antibody (M12622). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM50A antigen affinity purified monoclonal antibody (Catalog # M12622) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM50A at approximately 40 kDa. The expected band size for FAM50A is at 40 kDa.
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ReactivityHuman, Mouse, Rat
TargetFAM50A
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