Anti-Fascin Antibody [JM12-53]

HUABIO

Anti-Fascin Antibody [JM12-53]

7

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

WB,IF-Cell,IF-Tissue,IHC-P,FC,IP

Research Use Only

Monoclonal

JM12-53

1 mg/ml

Unconjugated

FSCN1

fascin actin-bundling protein 1

FAN1, HSN, SNL, p55, fascin, 55 kDa actin-bundling protein, epididymis secretory sperm binding protein, fascin homolog 1, actin-bundling protein, singed-like (fascin homolog, sea urchin)

Rabbit

IgG

Fascin

Fascin is an actin bundling protein. Fascin binds beta-catenin,and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. Fascin localizes to actin-rich protrusions at the cell surface called filopodia. T regulatory cell adhesion to antigen presenting dendritic cell causes sequestration of Fascin-1, an actin-bundling protein essential for immunological synapse formation, and skews Fascin-1-dependent actin polarization in antigen presenting dendritic cells toward the T reg cell adhesion zone. Although it is reversible upon T regulatory cell disengagement, this sequestration of essential cytoskeletal components causes a lethargic state of dendritic cells, leading to reduced T cell priming. This suggests Treg-mediated suppression of antigen presenting cells is a multi-step process. In addition to CTLA-4 CD80/CD86 interaction fascin dependent polarization of cytoskeleton towards dendritic cell Treg immune synapse play a pivotal role. In normal tissue, inflammation and the immune response would be limited by secretion of TGF-beta. TGF-beta on the one hand induces fascin expression, but on the other hand, restricts activity of transcription factor NF-kappaB. This results to limited fascin expression and allows tissue to rebuild epithelial barriers. In cancer, instead, TGF-beta does not restrict NF-kappaB activity, and both can increase fascin expression, disrupting tissue structure and function.

Human, Mouse, Rat

Human,Mouse,Rat

-20°C,2°C to 8°C

41116161