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Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Anti-FERMT2 Antibody

HPA040505
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFERMT2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FERMT2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10979
  • Target name
    FERMT2
  • Target description
    FERM domain containing kindlin 2
  • Target synonyms
    KIND2, MIG2, PLEKHC1, UNC112, UNC112B, mig-2, fermitin family homolog 2, PH domain-containing family C member 1, fermitin family member 2, kindlin 2, mitogen inducible gene 2 protein, mitogen inducible gene-2, pleckstrin homology domain containing, family C (with FERM domain) member 1, pleckstrin homology domain containing, family C member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96AC1
  • Protein Name
    Fermitin family homolog 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Kindlin 2/FERMT2 using anti-Kindlin 2/FERMT2 antibody (A03653-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kindlin 2/FERMT2 antigen affinity purified polyclonal antibody (Catalog # A03653-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kindlin 2/FERMT2 at approximately 70 kDa. The expected band size for Kindlin 2/FERMT2 is at 70 kDa.
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