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Immunohistochemical staining of human hypothalamus shows moderate positivity in processes.
Immunohistochemical staining of human hypothalamus shows moderate positivity in processes.
Immunohistochemical staining of human hypothalamus shows moderate positivity in processes.

Anti-FEZF1 Antibody

HPA073693
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFEZF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FEZF1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID389549
  • Target name
    FEZF1
  • Target description
    FEZ family zinc finger 1
  • Target synonyms
    FEZ, HH22, ZNF312B, fez family zinc finger protein 1, zinc finger protein 312B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA0PJY2
  • Protein Name
    Fez family zinc finger protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FEZF1 using anti-FEZF1 antibody (A09653-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FEZF1 antigen affinity purified polyclonal antibody (Catalog # A09653-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FEZF1 at approximately 65KD. The expected band size for FEZF1 is at 65KD.
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