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Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.

Anti-FGF7 Antibody

HPA043605
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFGF7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FGF7
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2252
  • Target name
    FGF7
  • Target description
    fibroblast growth factor 7
  • Target synonyms
    HBGF-7, KGF, fibroblast growth factor 7, FGF-7, heparin-binding growth factor 7, keratinocyte growth factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21781
  • Protein Name
    Fibroblast growth factor 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of KGF/FGF7 using anti-KGF/FGF7 antibody (A01931-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KGF/FGF7 antigen affinity purified polyclonal antibody (Catalog # A01931-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KGF/FGF7 at approximately 22KD. The expected band size for KGF/FGF7 is at 22KD.
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