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ProductsBack/Anti-Fibrin beta-chain [8E5]

Anti-Fibrin beta-chain [8E5]

Ab00799-10.0
Absolute Antibody
ApplicationsELISA, Other Application
Product group Antibodies
TargetFGB
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Fibrin beta-chain [8E5]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The recombinant scFv version of 8E5 retains the same antigen-binding activity of the parent antibody. 8E5 can bind to fibrin in the monomeric and polymeric form, both in vivo and ex vivo. This antibody can also be used for the immunodetection of fibrin in vivo. Standard ELISA and competitive ELISA using the epitope peptide has been conducted with both the parental antibody and the scFv.
  • Applications
    ELISA, Other Application
  • Applications Supplier
    ELISA; in vivo; ex vivo; imaging
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8E5
  • Gene ID2244
  • Target name
    FGB
  • Target description
    fibrinogen beta chain
  • Target synonyms
    beta-fibrinogen; epididymis secretory sperm binding protein Li 78p; fibrinogen beta chain; fibrinogen, B beta polypeptide; HEL-S-78p
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP02675
  • Protein Name
    Fibrinogen beta chain
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic bèta-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Fibrinogen beta chain/FGB using anti-Fibrinogen beta chain/FGB antibody (A01204-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrinogen beta chain/FGB antigen affinity purified polyclonal antibody (Catalog # A01204-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrinogen beta chain/FGB at approximately 56 kDa. The expected band size for Fibrinogen beta chain/FGB is at 56 kDa.
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