Anti-Fibrin beta-chain [8E5]

Absolute Antibody

Anti-Fibrin beta-chain [8E5]

7

8E5 binds to the amino-terminal region of the beta-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. The epitope recognised by this clone is only accessible upo

The recombinant scFv version of 8E5 retains the same antigen-binding activity of the parent antibody. 8E5 can bind to fibrin in the monomeric and polymeric form, both in vivo and ex vivo. This antibody can also be used for the immunodetection of fibrin in vivo. Standard ELISA and competitive ELISA using the epitope peptide has been conducted with both the parental antibody and the scFv.

ELISA, Other Application

ELISA; in vivo; ex vivo; imaging

Research Use Only

Monoclonal

8E5

FGB

fibrinogen beta chain

beta-fibrinogen; epididymis secretory sperm binding protein Li 78p; fibrinogen beta chain; fibrinogen, B beta polypeptide; HEL-S-78p

Mouse

IgG1

Fibrinogen beta chain

Human

Human

8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic bèta-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).

-20°C,2°C to 8°C

12352203