Anti-Flavocytochrome B2 [B2B4]

Absolute Antibody

Anti-Flavocytochrome B2 [B2B4]

9

The antibody recognized a conformational epitope on the Heme-binding domain of Flb2 (residues 1-99). Specifically, the epitope consists of Asn30, residues 63 to 65 (GluPro-Leu) and 67 to 70 (Ala-Pro-Asn-Val), Asp72, Tyr74, and the two heme propionate grou

ELISA indicated that the antibody, either the IgG or the Fab fragment, was able to recognize the heme-binding domain of flavocytochrome b2. (PMID: 8674650) For the Fab fragment, Kd values of 80 nM and 30 nM were determined in ELISA competition experiments and fluorescence quenching titrations, respectively. (PMID: 9521665)

ELISA

Research Use Only

Monoclonal

B2B4

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Yeast

-20°C,2°C to 8°C

12352203