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Anti-FOXN1 Antibody

Anti-FOXN1 Antibody

A28232
Antibodies.com
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-FOXN1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    >95%
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to FOXN1
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Lane 1:rat thymus lysates probed with Rabbit Anti-FOXN1 Polyclonal Antibody, Unconjugated (bs-6970R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
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Western Blot Positive WB detected in: Raji whole cell lysate, Mouse lung tissue, Mouse stomach tissue All lanes: FOXN1 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69 kDa Observed band size: 69 kDa
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Figure 1. Western blot analysis of FOXN1 using anti-FOXN1 antibody (A03369-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXN1 antigen affinity purified polyclonal antibody (Catalog # A03369-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXN1 at approximately 69KD. The expected band size for FOXN1 is at 69KD.
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