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Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Anti-FOXP2 Antibody

HPA000382
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFOXP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FOXP2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID93986
  • Target name
    FOXP2
  • Target description
    forkhead box P2
  • Target synonyms
    CAGH44, SPCH1, TNRC10, forkhead box protein P2, CAG repeat protein 44, forkhead/winged-helix transcription factor, trinucleotide repeat containing 10, trinucleotide repeat-containing gene 10 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15409
  • Protein Name
    Forkhead box protein P2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of FOXP2 using anti-FOXP2 antibody (A01329-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate,Lane 3: human SK-OV-3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXP2 antigen affinity purified polyclonal antibody (Catalog # A01329-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXP2 at approximately 90KD. The expected band size for FOXP2 is at 80KD.
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