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Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Anti-FRY Antibody

HPA041635
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFRY
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FRY Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10129
  • Target name
    FRY
  • Target description
    FRY microtubule binding protein
  • Target synonyms
    13CDNA73, 214K23.2, C13orf14, CG003, bA207N4.2, bA37E23.1, protein furry homolog, WUGSC:H_2G3A.1, furry homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5TBA9
  • Protein Name
    Protein furry homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of FRY using anti-FRY antibody (A01003). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat lung tissue lysate, Lane 5: mouse brain tissue lysate, Lane 6: mouse lung tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRY antigen affinity purified polyclonal antibody (Catalog # A01003) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRY at approximately 339 kDa. The expected band size for FRY is at 339 kDa.
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