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Anti-FSCN1 [SAIC-32C-205]

Ab00325-1.1
Absolute Antibody
ApplicationsMass Spectrometry
Product group Antibodies
ReactivityHuman
TargetFSCN1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-FSCN1 [SAIC-32C-205]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The original characterization data for this antibody can be found here.
  • Applications
    Mass Spectrometry
  • Applications Supplier
    immunoMRM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SAIC-32C-205
  • Gene ID6624
  • Target name
    FSCN1
  • Target description
    fascin actin-bundling protein 1
  • Target synonyms
    FAN1, HSN, SNL, p55, fascin, 55 kDa actin-bundling protein, epididymis secretory sperm binding protein, fascin homolog 1, actin-bundling protein, singed-like (fascin homolog, sea urchin)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ16658
  • Protein Name
    Fascin
  • Scientific Description
    This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Peptide "TVEEAENIAVTSGVVR" derived from Fascin, which organises filamentous actin into bundles, conjugated to KLH.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Fascin/FSCN1 using anti-Fascin/FSCN1 antibody (PB9592). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fascin/FSCN1 antigen affinity purified polyclonal antibody (Catalog # PB9592) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fascin/FSCN1 at approximately 55 kDa. The expected band size for Fascin/FSCN1 is at 55 kDa.
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