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Immunohistochemical staining of human pituitary gland shows moderate to strong cytoplasmic positivity in glandular cells in anterior lobe.
Immunohistochemical staining of human pituitary gland shows moderate to strong cytoplasmic positivity in glandular cells in anterior lobe.
Immunohistochemical staining of human pituitary gland shows moderate to strong cytoplasmic positivity in glandular cells in anterior lobe.

Anti-FSHB Antibody

HPA069703
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFSHB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FSHB
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2488
  • Target name
    FSHB
  • Target description
    follicle stimulating hormone subunit beta
  • Target synonyms
    HH24, follitropin subunit beta, FSH-B, FSH-beta, follicle stimulating hormone beta subunit, follicle stimulating hormone, beta polypeptide, follitropin, beta chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01225
  • Protein Name
    Follitropin subunit beta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of FSH beta using anti-FSH beta antibody (PB9106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours Lane 1: recombinant human FSH beta protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSH beta antigen affinity purified polyclonal antibody (Catalog # PB9106) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSH beta at approximately 36 kDa. The expected band size for FSH beta is at 36 kDa.
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