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Immunohistochemical staining of human placenta shows strong nuclear positivity in a subset of trophoblastic cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in a subset of trophoblastic cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in a subset of trophoblastic cells.

Anti-FSTL3 Antibody

HPA045378
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFSTL3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FSTL3
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10272
  • Target name
    FSTL3
  • Target description
    follistatin like 3
  • Target synonyms
    FLRG, FSRP, follistatin-related protein 3, follistatin-like 3 (secreted glycoprotein), follistatin-like protein 3, follistatin-related gene protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95633
  • Protein Name
    Follistatin-related protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FSTL3 using anti-FSTL3 antibody (PA1915). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTL3 antigen affinity purified polyclonal antibody (Catalog # PA1915) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSTL3 at approximately 22-24KD. The expected band size for FSTL3 is at 22KD.
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