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Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.

Anti-FXR1 Antibody

HPA018246
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFXR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FXR1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8087
  • Target name
    FXR1
  • Target description
    FMR1 autosomal homolog 1
  • Target synonyms
    fragile X mental retardation syndrome-related protein 1; fragile X mental retardation, autosomal homolog 1; FXR1P; MYOPMIL; MYORIBF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51114
  • Protein Name
    Fragile X mental retardation syndrome-related protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate All lanes: FXR1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 70, 61, 60 kDa Observed band size: 70 kDa
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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
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Figure 1. Western blot analysis of FXR1 using anti-FXR1 antibody (A03308-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey skeletal muscle tissue lysates, Lane 4: monkey heart tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXR1 antigen affinity purified polyclonal antibody (Catalog # A03308-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FXR1 at approximately 80 kDa. The expected band size for FXR1 is at 80 kDa.
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