Figure 1. Western blot analysis of FXR2 using anti-FXR2 antibody (A05130-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: huamn U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXR2 antigen affinity purified polyclonal antibody (Catalog # A05130-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FXR2 at approximately 93 kDa. The expected band size for FXR2 is at 74 kDa.
Figure 1. Western blot analysis of FXR2 using anti-FXR2 antibody (A05130-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: huamn U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXR2 antigen affinity purified polyclonal antibody (Catalog # A05130-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FXR2 at approximately 93 kDa. The expected band size for FXR2 is at 74 kDa.
Anti-FXR2 Antibody Picoband(r)
A05130-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetFXR2
Overview
- SupplierBoster Bio
- Product NameAnti-FXR2 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID9513
- Target nameFXR2
- Target descriptionFMR1 autosomal homolog 2
- Target synonymsFMR1L2; fragile X mental retardation syndrome-related protein 2; fragile X mental retardation, autosomal homolog 2; fragile X-mental retardation 1-like 2; FXR2P
- HostRabbit
- IsotypeIgG
- Protein IDP51116
- Protein NameFragile X mental retardation syndrome-related protein 2
- Scientific DescriptionBoster Bio Anti-FXR2 Antibody Picoband® catalog # A05130-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Monkey, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203