Anti-G [HENV-26], Human IgG1, Fc Silent(tm), lambda

Absolute Antibody

Anti-G [HENV-26], Human IgG1, Fc Silent(tm), lambda

9

The antibody is specific for RBP of HeV and NiV. The antibody epitope overlaps with the receptor binding sites of RBP.

The specificity of the original format of the antibody for recombinant RBP head domain proteins from HeV, NiVM, or NiVB was confirmed by ELISA analysis (EC50 = 0.14,0.09 and 0.07 ug/mL respectively). The antibody could neutralize HeV, NiVB and NiVM in In vitro neutralization assays (IC50= 0.07, 0.03 and 0.04 ug/mL respectively). The antibody could bind to the full-length RBPs by flow cytometry. The EC50 values for binding of the antibody ranged from 325 to 343 ng/mL for binding to HeV, NiVM, or NiVB. The kinetics of binding of the antibody to RBPs was measured on a biosensor to determine affinity (KD= 2.9, 2.2, or 1.0 nM for HeV, NiVM, or NiVB, respectively). The crystal structures of Fab fragment of the antibody in complex with both HeV-RBP and NiV-RBP were determined. The antibody protected ferrets in lethal models of infection with NiV (Dong et al., 2020; PMID: 33306954).

Flow Cytometry, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

HENV-26

G

attachment glycoprotein

attachment glycoprotein; Nvgp5

Human

IgG1

Glycoprotein G

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203