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Immunohistochemical staining of rat hippocampal formation shows strong positivity in GABAergic fibers in the CA1 area.
Immunohistochemical staining of rat hippocampal formation shows strong positivity in GABAergic fibers in the CA1 area.
Immunohistochemical staining of rat hippocampal formation shows strong positivity in GABAergic fibers in the CA1 area.

Anti-GAD1 Antibody

AMAB91079
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetGAD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GAD1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL2919
  • Conjugate
    Unconjugated
  • Gene ID2571
  • Target name
    GAD1
  • Target description
    glutamate decarboxylase 1
  • Target synonyms
    67 kDa glutamic acid decarboxylase; CPSQ1; DEE89; GAD; GAD-67; glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kDa); SCP
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ99259
  • Protein Name
    Glutamate decarboxylase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAD67/GAD1 using anti-GAD67/GAD1 antibody (PB9183). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD67/GAD1 antigen affinity purified polyclonal antibody (Catalog # PB9183) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD67/GAD1 at approximately 67 kDa. The expected band size for GAD67/GAD1 is at 67 kDa.
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