Figure 1. Western blot analysis of GADD34/Ppp1r15a using anti-GADD34/Ppp1r15a antibody (A02394-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD34/Ppp1r15a antigen affinity purified polyclonal antibody (Catalog # A02394-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD34/Ppp1r15a at approximately 100 kDa. The expected band size for GADD34/Ppp1r15a is at 73 kDa.
Figure 1. Western blot analysis of GADD34/Ppp1r15a using anti-GADD34/Ppp1r15a antibody (A02394-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD34/Ppp1r15a antigen affinity purified polyclonal antibody (Catalog # A02394-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD34/Ppp1r15a at approximately 100 kDa. The expected band size for GADD34/Ppp1r15a is at 73 kDa.
Anti-GADD34/Ppp1r15a Antibody Picoband(r)
A02394-4-HRP
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetPpp1r15a
Overview
- SupplierBoster Bio
- Product NameAnti-GADD34/Ppp1r15a Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID17872
- Target namePpp1r15a
- Target descriptionprotein phosphatase 1, regulatory subunit 15A
- Target synonyms9630030H21; Gadd3; Gadd34; growth arrest and DNA damage-inducible protein GADD34; growth arrest and DNA-damage-inducible 34; Myd116; myeloid differentiation primary response gene 116; myeloid differentiation primary response protein MyD116; protein phosphatase 1 regulatory subunit 15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A
- HostRabbit
- IsotypeIgG
- Protein IDP17564
- Protein NameProtein phosphatase 1 regulatory subunit 15A
- Scientific DescriptionBoster Bio Anti-GADD34/Ppp1r15a Antibody Picoband® catalog # A02394-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203