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Anti-Galectin-4 [FF04]

AB03294-1.1-BT
Absolute Antibody
ApplicationsImmunoFluorescence, Other Application
Product group Antibodies
TargetLGALS4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Galectin-4 [FF04]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Immunofluorescence analysis on the human colorectal adenocarcinoma cell line LS174T was performed in order to verify the ability of the scFv fragment to recognize the natively expressed galectin-4 antigen. The antibody showed strong cytoplasmic staining. The binding affinity of the scFv fragment to human Galectin-4 was measured by surface plasmon resonance (Kd= 2.44x10-7 M) (US20190309060).
  • Applications
    ImmunoFluorescence, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FF04
  • Gene ID3960
  • Target name
    LGALS4
  • Target description
    galectin 4
  • Target synonyms
    antigen NY-CO-27; GAL4; gal-4; galectin-4; L-36 lactose-binding protein; L36LBP; lactose-binding lectin 4; lectin, galactoside-binding, soluble, 4
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP56470
  • Protein Name
    Galectin-4
  • Scientific Description
    This full-length chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAL4 using anti-GAL4 antibody (PB9952). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates, Lane 2: COLO320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAL4 antigen affinity purified polyclonal antibody (Catalog # PB9952) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAL4 at approximately 39 kDa. The expected band size for GAL4 is at 36 kDa.
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