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Immunohistochemical staining of human liver shows weak to moderate cytoplasmic positivity in hepatocytes and moderate staining of bile canaliculi.
Immunohistochemical staining of human liver shows weak to moderate cytoplasmic positivity in hepatocytes and moderate staining of bile canaliculi.
Immunohistochemical staining of human liver shows weak to moderate cytoplasmic positivity in hepatocytes and moderate staining of bile canaliculi.

Anti-GAMT Antibody

HPA051806
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetGAMT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GAMT
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2593
  • Target name
    GAMT
  • Target description
    guanidinoacetate N-methyltransferase
  • Target synonyms
    CCDS2; epididymis secretory protein Li 20; guanidinoacetate N-methyltransferase; HEL-S-20; PIG2; TP53I2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14353
  • Protein Name
    Guanidinoacetate N-methyltransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAMT using anti-GAMT antibody (A02886-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAMT antigen affinity purified polyclonal antibody (Catalog # A02886-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAMT at approximately 26 kDa. The expected band size for GAMT is at 26 kDa.
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