Anti-gangliosides [AKH11 ], Human IgG1, kappa

Absolute Antibody

Anti-gangliosides [AKH11 ], Human IgG1, kappa

9

The specificity of the original antibody (human IgM) was assessed using ELISA, revealing that it reacted with gangliosides in the following order: GM3 (N-acetylneuraminic acid) > GT1b > GD3 > GD1a > GQ1b. A recombinant version of the antibody was generated, and its biological activity was evaluated. CDC activity tests demonstrated efficacy against MeWo, M12, M14, and G-361 melanoma cells, while showing no significant CDC activity against human normal skin fibroblasts, human adenocarcinoma, human neuroblastoma, or Vero cells. These findings confirm that the recombinant antibody retains the biological activities of the original AKH11 antibody (EP0492409A1).

Functional Assay, ELISA

Research Use Only

Monoclonal

AKH11

Human

IgG1

This reformatted human antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

41116161