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Anti-GAPR1 (N-term) Antibody

102-23068
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetGLIPR2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GAPR1 (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB25221
  • Gene ID152007
  • Target name
    GLIPR2
  • Target description
    GLI pathogenesis related 2
  • Target synonyms
    C9orf19, GAPR-1, GAPR1, gliPR 2, Golgi-associated plant pathogenesis-related protein 1, 17kD fetal brain protein, glioma pathogenesis-related protein 2, golgi-associated PR-1 protein
  • Host
    Rabbit
  • Protein IDQ9H4G4
  • Protein Name
    Golgi-associated plant pathogenesis-related protein 1
  • Scientific Description
    Rabbit Anti-GAPR1 (N-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLIPR2 using anti-GLIPR2 antibody (A10232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLIPR2 antigen affinity purified polyclonal antibody (Catalog # A10232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLIPR2 at approximately 17 kDa. The expected band size for GLIPR2 is at 17 kDa.
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Western blot analysis in human cell lines U2OS and Caco-2 using Anti-GLIPR2 antibody. Corresponding GLIPR2 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
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