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Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles.

Anti-GAS1 Antibody

HPA036085
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetGAS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GAS1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2619
  • Target name
    GAS1
  • Target description
    growth arrest specific 1
  • Target synonyms
    GAS-1; Growth arrest-specific gene-1; growth arrest-specific protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54826
  • Protein Name
    Growth arrest-specific protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GAS1 using anti-GAS1 antibody (A06815-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS1 antigen affinity purified polyclonal antibody (Catalog # A06815-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAS1 at approximately 40 kDa. The expected band size for GAS1 is at 40 kDa.
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