Anti-Gastrin [XPA067.06]

Absolute Antibody

Anti-Gastrin [XPA067.06]

9

To determine the efficacy of gastrin neutralization by these antibodies in vitro, a flow cytometric measurement of intracellular calcium flux was performed using the human IgG1 version of this antibody on a CCK2R overexpressing cell line (AGS-TR). This antibody showed a 70%-80% neutralization in Ca flux in cells stimulated with gastrin (US8278421) (Hsu et al, 2008; pmid:18589401). Further the binding affinity of the human version of this antibody was determined using surface plasmon resonance and affinity constants were calculated from the kinetic rate constants for antibody binding to biotinylated peptide immobilized on a Streptavidin sensor chip (US8278421) (Hsu et al, 2008; pmid:18589401). The human version of this antibody has shown the ability to increase the stomach pH of mice to pH 4 in combination with human gastrin and famotidine. The pH with only human gastrin and famotidine was pH 2.5 (US8278421) (Hsu et al, 2008; pmid:18589401). Screening of gastrin positive antibody clones was done using ELISA (Hsu et al, 2008; pmid:18589401). Inhibition of gastrin activity has potential for therapeutic use as a suppressor of acid secretion as well as an inhibitor of gastrin-responsive tumors (Hsu et al, 2008; pmid:18589401).

Functional Assay, Flow Cytometry, ELISA

Research Use Only

Monoclonal

XPA067.06

GAST

gastrin

GAS; gastrin; preprogastrin

Human

IgG1

Gastrin

This full-length reformatted human antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203