Anti-gB [1G2], Human IgG3, lambda

Absolute Antibody

Anti-gB [1G2], Human IgG3, lambda

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This antibody is specific for a surface epitope in antigenic domain 5 (AD-5), also termed Domain I (Dom I), of Human cytomegalovirus (hCMV) (strain Towne), also called Human herpesvirus 5 (HHV-5). Dom I resides between amino acid residues 133 to 343 of th

The original version of the antibody was used in a neutralization assay against human cytomegalovirus (hCMV). It was found to neutralize hCMV with high potency - it neutralized the virus with an IC50 of 0.1 microg/ml and an IC90 of 0.4 microg/ml (US9346874B2). The Fab version of this antibody was generated and its structure in complex with hCMV gB was explored using crystallography. It was also used in neutralization assays, surface staining of full-length gB in flow cytometry, and gB immunoprecipitation (Chandramouli et al., 2015; PMID: 26365435).

Flow Cytometry, ImmunoPrecipitation, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

1G2

Human

IgG3

Envelope glycoprotein B

Virus

-20°C,2°C to 8°C

12352203